Guidelines for Certified User for LSM 510

The following are prerequisites for becoming a certified user.

1. Submission of a letter from P.I. requesting User Certification.
2. Solid working experience using fluorescence on a research microscope.
3. To study the LSM 510 manual. A copy of the manual is posted on the spine.rutgers.edu website.
4. At least 6 hours of hands-on training with Confocal Administrator on the LSM 510.

· Minimum 4 hours of training with handling of our fluorescence microscope Axiovert 100 and LSM 510 operation.

· Minimum 2 hours of supervised operation after the required training.

· Special attention will be paid to the ability of the candidate to follow practices related to personal safety, equipment integrity and data base preservation.

5. User Certification will be obtained after Confocal Administrator and Center Director jointly agree that the Candidate has achieved sufficient proficiency.

The following are guidelines for Confocal microscope usage.

1. Users must fill out User Qualification Form* and provide an account number.
2. Users must sign in and off the User Logbook to record their usage.
3. Users must immediately report to a Designated Staff User or Confocal Administrator (Noriko Kane-Goldsmith, 732-445-2061, ext. 40110) of the W.M. Keck Center for any irregularity in the use or function of the LSM 510 and related instruments.
4. Lasers will be turned on and off by the Designated Staff Users and Confocal Administrator only.

* Will be posted on the http://spine.rutgers.edu/if/ website.