Animals pooled for Affymetrix Experiments

Since injured spinal cord segments yield lesser quantities of RNA, and since 10 mg of RNA was required for each Affymetrix target preparation, animals were pooled without regard to sex.  The D1 segment had less cell loss due to injury and therefore provided sufficient RNA to preclude pooling. The table below lists specific animal numbers and sexes pooled.

I Segment
Sample Hrs Post Injury Group* Number of animals Animal codes Sexes
0 0 A 3 66-68 M, M, M
0A 0 B 2 92, 94 F, M
0B 0 C 2 93, 95 F, M
6A 6 A 2 97, 99 F, M
6B 6 B 1 98 F
6C 6 -- 3 75-77 F, F, F
6D 6 C 1 107 M
12 12 A 3 84-86 M, M, M
12B 12 B 2 104, 105 F, F
12C 12 C 1 106 F
24 24 A 3 69-71 M, M, M
24A 24 B 2 100, 103 M, F
24B 24 C 2 101, 102 M, F
48 48 A 3 72-74 M, M, M
48B 48 B 2 HT70, HT71 M, M
48C 48 C 2 HT58, HT59 M, M
D1 Segment
Sample Hrs Post Injury Group* Number of animals Animal codes Sexes
0A 0 A 1 92 F
0C 0 B 1 94 M
0D 0 C 1 95 M
6A 6 A 1 96 M
6B 6 B 1 98 F
6C 6 C 1 99 F
12A 12 A 1 104 F
12B 12 B 1 105 F
12C 12 C 1 106 F
24A 24 A 1 101 M
24B 24 B 1 102 F
24C 24 C 1 103 F
48A 48 A 1 HT 70 M
48B 48 B 1 HT 71 M
48C 48 C 1 HT 58 M

*Group letter matches downloadable data table.